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Animal Reproduction and Physiology
Asian-Australasian Journal of Animal Sciences 1993;6(2): 319-323.
https://doi.org/10.5713/ajas.1993.319    Published online June 1, 1993.
Effects of trypsin-digested bovine growth hormone on whole-body protein synthesis in vitro in chicken embryos
K. Kita, S. Hatano, J. Okumura, T. Muramatsu
Abstract
The effect of bovine growth hormone digested with trypsin on whole-body protein synthesis in vitro of chicken embryos was investigated by using a whole-embryo culture system. Bovine growth hormone at 5.3 and 530 ng/ml was digested partially and completely with trypsin for 4 min and 18 h, respectively. After culturing chicken embryos with a synthetic medium containing L-[4-3H] pheylalanine, whole-embryo protein synthesis was determined from the ratio of specific radioactivities of free and protein-bound pheylalanine. Whole-embryo protein synthesis of the control group cultured with no bovine growth hormone was 49.5 2.2 %/d. There was no significant interaction between digestion time and the concentration of trypsin-digested bovine growth hormone. Tryptic digestion of bovine growth hormone increased fractional synthesis rates of whole-body protein compared to the 0-min groups, and there was no significant difference between the 4-min and 18-h groups. The higher concentration (530 ng/ml) of trypsin-digested bovine growth hormone was more effective in enhancing whole-embtyo protein synthesis than the lower concentration (5.3 ng/ml).
Keywords: Protein Synthesis; Bovine Growth Hormone; Trypsin Digestion; Embryo; Chicken
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