Go to Top Go to Bottom
Article
Animal Reproduction and Physiology
Asian-Australasian Journal of Animal Sciences 2009;22(2): 187-193.
https://doi.org/10.5713/ajas.2009.80324    Published online January 6, 2009.
Isolation and In vitro Culture of Pig Spermatogonial Stem Cell
Su Young Han, Mukesh Kumar Gupta, Sang Jun Uhm, Hoon Taek Lee*
Correspondence:  Hoon Taek Lee,
Abstract
The present study identified the favorable conditions for isolation, enrichment and in vitro culture of highly purified, undifferentiated pig spermatogonial stem cell (SSC) lines that proliferate for long periods of time in culture. The colonies displayed morphology similar to miceSSC and were positive for markers of SSC (PGP9.5), proliferating germ cell (PigVASA), pre-meiotic germ cell (DAZL) and pluripotency (OCT4, SSEA-1, NANOG, and SOX2) based on immuno-cytochemistry and RT-PCR. The purity of these colonies was confirmed by negative expression of markers for sertoli cell (GATA4 and SOX9), peritubular myoid cell (-SMA), differentiating spermatogonial and germ cells (c-KIT). The colonies could be maintained with undifferentiated morphology for more than two months and passaged more than 8 times with doubling time between 6-7 days. Taken together, we conclude that pigSSC could be successfully isolated and cultured in vitro and they possess characteristics similar to miceSSC.
Keywords: Spermatogonial Stem Cell; SSC; Pluripotency; Long Term Culture; Pig
TOOLS
METRICS Graph View
  • 18 Crossref
  • 21 Scopus
  • 5,461 View
  • 94 Download
Related articles
Editorial Office
Asian-Australasian Association of Animal Production Societies(AAAP)
Room 708 Sammo Sporex, 23, Sillim-ro 59-gil, Gwanak-gu, Seoul 08776, Korea   
TEL : +82-2-888-6558    FAX : +82-2-888-6559   
E-mail : animbiosci@gmail.com               

Copyright © 2025 by Asian-Australasian Association of Animal Production Societies.

Developed in M2PI

Close layer
prev next