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The Effect of Melatonin on Mitochondrial Function in Endotoxemia Induced by Lipopolysaccharide |
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Jing Liu, Fengming Wu, Yuqing Liu, Tao Zhang, Zhaoxin Tang |
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| Abstract |
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This study examined the metabolism of free radicals in hepatic mitochondria of goats induced by lipopolysaccharide (LPS), and investigated the effects of melatonin (MT). Forty-eight healthy goats (101.2 kg) were randomly selected and divided into four groups: saline control, LPS, MT+LPS and MT. The goats within each group were3 sacrificed either 3 or 6 h after treatment and the livers removed to isolate mitochondria. The respiration control ratio (RCR), the ADP:O ratio, the oxidative phosphorylation ratio (OPR), the concentration of H2O2 and the activities of Complex I-IV were determined. The mitochondrial membrane potential (∆m) was analyzed by flow cytometry. The results showed that RCR, O/P and OPR of the LPS group decreased (p<0.05), as well as activities of respiratory complexes, whereas the generation of H2O2 in Complex III increased (p<0.05) after 3 h, while Complex II and III increased after 6 h. Also, it was found that the mitochondrial membrane potential of the LPS group declined (p<0.05). However, pre-treatment with MT attenuated the injury induced by LPS, which not only presented higher (p<0.05) RCR, O/P, OPR, and respiratory complex activities, but also maintained the ∆m. Interestingly, it is revealed that, in the MT+LPS group, the generation of H2O2 increased firstly in 3 h, and then significantly (p<0.05).decreased after 6 h. In the MT group, the function of mitochondria, the transmenbrane potential and the generation of H2O2 were obviously improved compared to the control group. Conclusion: melatonin prevents damage caused by LPS on hepatic mitochondria of goats. |
| Keywords:
Goat; Endotoxemia; Mitochondrial Function; Free Radicals; Melatonin |
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