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Association of single-nucleotide polymorphisms in dual specificity phosphatase 8 and insulin-like growth factor 2 genes with inosine-5′-monophosphate, inosine, and hypoxanthine contents in chickens |
Jean Pierre Munyaneza1 
, Minjun Kim1
, Eunjin Cho2
, Aera Jang3
, Hyo Jun Choo4,*
, Jun Heon Lee1,2,*
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1Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Korea
2Department of Bio-AI Convergence, Chungnam National University, Daejeon 34134, Korea
3Department of Applied Animal Science, College of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea
4Poultry Research Institute, National Institute of Animal Science, Pyeongchang 25342, Korea |
Correspondence:
Hyo Jun Choo, Tel: +82-10-2647-2888, Fax: +82-33-330-9529, Email: hyojy@korea.kr
Jun Heon Lee, Tel: +82-42-821-5779, Fax: +82-42-825-9754, Email: junheon@cnu.ac.kr
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Received: 8 March 2023 • Revised: 21 April 2023 • Accepted: 22 May 2023 |
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| Abstract |
Objective
This study aimed to identify the single-nucleotide polymorphisms (SNPs) in the dual-specificity phosphatase 8 (DUSP8) and insulin-like growth factor 2 (IGF2) genes and to explore their effects on inosine-5′-monophosphate (IMP), inosine, and hypoxanthine contents in Korean native chicken -red-brown line (KNC-R Line).
Methods
A total sample of 284 (males, n = 127; females n = 157) and 230 (males, n = 106; females, n = 124) aged of 10 weeks old KNC-R line was used for genotyping of DUSP8 and IGF2 genes, respectively. One SNP (rs313443014 C>T) in DUSP8 gene and two SNPs (rs315806609A/G and rs313810945T/C) in IGF2 gene were used for genotyping by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and KASP methods, respectively. The Two-way analysis of variance of the R program was used to associate DUSP8 and IGF2 genotypes with nucleotide contents in KNC-R chickens.
Results
The DUSP8 (rs313443014 C>T) was polymorphic in KNC-R line and showed three genotypes: CC, CT, and TT. The IGF2 gene (rs315806609A/G and rs313810945T/C) was also polymorphic and had three genotypes per SNP, including GG, AG, and AA for the SNP rs315806609A/G and genotypes: CC, CT, and TT for the SNP rs313810945T/C. Association resulted into a strong significant association (p<0.01) with IMP, inosine, and hypoxanthine. Moreover, the significant effect of sex (p<0.05) on nucleotide content was also observed.
Conclusion
The SNPs in the DUSP8 and IGF2 genes might be used as genetic markers in the selection and production of chickens with highly flavored meat. |
| Keywords:
Dual-specificity Phosphatase 8 (DUSP8); Insulin-like Growth Factor 2 (IGF2); Kompetitive Allele-specific Polymerase Chain Reaction (KASP); Korean Native Chicken; Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) |
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