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Impact of glucose and pyruvate on adenosine triphosphate production and sperm motility in goats |
Rangga Setiawan1,* 
, Raden Febrianto Christi1
, Ken Ratu Gharizah Alhuur1
, Rini Widyastuti1
, Nurcholidah Solihati1
, Siti Darodjah Rasad1
, Kundrat Hidajat1
, Duy Ngoc Do2
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1Department of Animal Production, Faculty of Animal Husbandry, Universitas Padjadjaran, Bandung-Sumedang KM 21, West Java 45363, Indonesia
2Department of Animal Science and Aquaculture, Dalhousie University, Truro, NS B2N 5E3, Canada |
Correspondence:
Rangga Setiawan, Tel: +62-821-6627-5237, Fax: +62-779-8241, Email: rangga.setiawan@unpad.ac.id
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Received: 19 June 2023 • Revised: 11 July 2023 • Accepted: 6 September 2023 |
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| Abstract |
Objective
This study evaluates goat sperm motility in response to metabolic substrates and various inhibitors, aiming to assess the relative contribution of glycolysis and mitochondrial oxidation for sperm movement and adenosine triphosphate (ATP) production.
Methods
In the present study, two main metabolic substrates; 0 to 0.5 mM glucose and 0 to 30 mM pyruvate were used to evaluate their contribution to sperm movements of goats. Using a 3-chloro-1,2-propanediol (3-MCPD), a specific inhibitor for glycolysis, and carbonyl cyanide 3-chlorophenylhydrazone as an inhibitor for oxidative phosphorylation, cellular mechanisms into ATP-generating pathways in relation to sperm movements and ATP production were observed. Data were analysed using one-way analysis of variance for multiple comparisons.
Results
Sperm motility analysis showed that either glucose or pyruvate supported sperm movement during 0 to 30 min incubation. However, the supporting effects were abolished by the addition of a glycolysis inhibitor or mitochondrial uncoupler, concomitant with a significant decrease in ATP production. Although oxidative phosphorylation produces larger ATP concentrations than those from glycolysis, sperm progressivity in relation to these two metabolic pathways is comparable.
Conclusion
Based on the present study, we suggest that goat sperm use glucose and pyruvate to generate cellular energy through glycolysis and mitochondrial respiration pathways to maintain sperm movement. |
| Keywords:
Adenosine Triphosphate; Glycolysis; Goat; Oxidative Phosphorylation; Sperm |
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