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Protective effect of Macleaya cordata isoquinoline alkaloids on lipopolysaccharide-induced liver injury in broilers |
Jiaxin Chen1 
, Weiren Yang2
, Hua Liu3
, Jiaxing Niu2
, Yang Liu2
, Qun Cheng1,*
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1Department of Animal Science, Qingdao Agricultural University, Qingdao 266109, China
2Key Laboratory of Efficient Utilization of Non-grain Feed Resources (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an 271018, China
3College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China |
Correspondence:
Qun Cheng, Tel: +86-15610089196, Email: chengqun@qau.edu.cn
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Received: 14 July 2023 • Revised: 29 August 2023 • Accepted: 18 September 2023 |
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| Abstract |
Objective
This experiment aimed to explore the protective action of dietary supplementation with isoquinoline alkaloids (IA) from Macleaya cordata on lipopolysaccharide (LPS)-induced liver injury in broilers.
Methods
Total 216 healthy broilers were selected in a 21-d trial and assigned randomly to the following 3 treatments: control (CON) group, LPS group, and LPS+IA group. The CON and LPS groups were provided with a basal diet, whereas the LPS+IA group received the basal diet supplemented with 0.6 mg/kg Macleaya cordata IA. Broilers in LPS and LPS+IA groups were intraperitoneally injected with LPS (1 mg/kg body weight) at 17, 19, and 21 days of age, while those in CON group were injected with equivalent amount of saline solution.
Results
Results showed LPS injection caused systemic and liver inflammation in broilers, inhibited immune function, and ultimately lead to liver injury. By contrast, supplementation of IA ameliorated LPS-induced adverse change in serum parameters, boosted immunity in LPS+IA group. Furthermore, IA suppressed the elevation of hepatic inflammatory cytokines and caspases levels induced by LPS, as well as the expressions of genes related to the tolllike receptor 4 (TLR4)/myeloid differentiation primary response 88 (MyD88)/nuclear factorkappa B (NF-κB) pathway.
Conclusion
Dietary inclusion of 0.6 mg/kg Macleaya cordata IA could enhance immune function of body and inhibit liver damage via inactivating TLR4/MyD88/NF-κB signaling pathway in broilers. |
| Keywords:
Broiler; Isoquinoline Alkaloids; Lipopolysaccharide; Liver Injury; Macleaya cordata |
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