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miR-133a-3p and miR-145-5p co-promote goat hair follicle stem cell differentiation by regulating NANOG and SOX9 expression |
Jian Wang1,2,a,* 
, Xi Wu1,a
, Liuming Zhang1
, Qiang Wang1
, Xiaomei Sun1
, Dejun Ji1
, Yongjun Li1,2,*
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1Key Laboratory of Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
2International Joint Research Laboratory in Universities of Jiangsu Province of China for Domestic Animal Germplasm Resources and Genetic Improvement, Yangzhou University, Yangzhou 225009, China |
Correspondence:
Jian Wang, Tel: +86-514-87992481, Fax: +86-514-87992481, Email: 008209@yzu.edu.cn
Yongjun Li, Tel: +86-514-87992481, Fax: +86-514-87992481, Email: liyj@yzu.edu.cn
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Received: 8 September 2023 • Revised: 3 October 2023 • Accepted: 20 October 2023
aThese authors contributed equally to this work. |
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| Abstract |
Objective
Hair follicle stem cells (HFSCs) differentiation is a critical physiological progress in skin hair follicle (HF) formation. Goat HFSCs differentiation is one of the essential processes of superior-quality brush hair (SQBH) synthesis. However, knowledge regarding the functions and roles of miR-133a-3p and miR-145-5p in differentiated goat HFSCs is limited.
Methods
To examine the significance of chi-miR-133a-3p and chi-miR-145-5p in differentiated HFSCs, overexpression and knockdown experiments of miR-133a-3p and miR-145-5p (Mimics and Inhibitors) separately or combined were performed. NANOG, SOX9, and stem cell differentiated markers (β-catenin, C-myc, Keratin 6 [KRT6]) expression levels were detected and analyzed by using real-time quantitative polymerase chain reaction, western blotting, and immunofluorescence assays in differentiated goat HFSCs.
Results
miR-133a-3p and miR-145-5p inhibit NANOG (a gene recognized in keeping and maintaining the totipotency of embryonic stem cells) expression and promote SOX9 (an important stem cell transcription factor) expression in differentiated stem cells. Functional studies showed that miR-133a-3p and miR-145-5p individually or together overexpression can facilitate goat HFSCs differentiation, whereas suppressing miR-133a-3p and miR-145-5p or both inhibiting can inhibit goat HFSCs differentiation.
Conclusion
These findings could more completely explain the modulatory function of miR-133a-3p and miR-145-5p in goat HFSCs growth, which also provide more understandings for further investigating goat hair follicle development. |
| Keywords:
miR-133a-3p; miR-145-5p; NANOG; SOX9; Stem Cell Differentiation |
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