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Antioxidant activity and metabolic regulation of sodium salicylate on goat sperm at low temperature |
Wenzheng Shen1,a 
, Yu Fu1,a
, Haiyu Bai1
, Zhiyu Zhang1
, Zhikun Cao1
, Zibo Liu1
, Chao Yang1
, Shixin Sun1
, Lei Wang1
, Chunhuan Ren1,2
, Yinghui Ling1,2
, Zijun Zhang1,2
, Hongguo Cao1,2,*
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1College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China
2Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Anhui Agricultural University, Hefei, 230036, China |
Correspondence:
Hongguo Cao,Email: caohongguo1@ahau.edu.cn
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Received: 30 August 2023 • Revised: 22 October 2023 • Accepted: 28 November 2023
aThese authors contributed equally to this work. |
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| Abstract |
Objective
The purpose of this study was to explore the effect of sodium salicylate (SS) on semen preservation and metabolic regulation in goats.
Methods
Under the condition of low temperature, SS was added to goat semen diluent to detect goat sperm motility, plasma membrane, acrosome, antioxidant capacity, mitochondrial membrane potential (MMP) and metabonomics.
Results
The results show that at the 8th day of low-temperature storage, the sperm motility of the 20 μM SS group was 66.64%, and the integrity rates of the plasma membrane and acrosome were both above 60%, significantly higher than those of the other groups. The activities of catalase and superoxide dismutase in the sperm of the 20 μM SS group were significantly higher than those of the control group, the contents of reactive oxygen species and malondialdehyde were significantly lower than those in the control group, the MMP was significantly higher than that in the control group, and the contents of Ca2+ and total cholesterol were significantly higher than those in the control group. Through metabonomics analysis, there were significant metabolic differences between the control group and the 20 μM SS group. Twenty of the most significant metabolic markers were screened, mainly involving five metabolic pathways, of which nicotinic acid and nicotinamide metabolic pathways were the most significant.
Conclusion
The results indicate that SS can effectively improve the low-temperature preservation quality of goat sperm. |
| Keywords:
Cryopreservation; Goat; Metabolism; Sodium Salicylate; Sperm Motility |
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