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Identification of new major histocompatibility complex-B Haplotypes in Bangladesh native chickens |
Thisarani Kalhari Ediriweera1 
, Prabuddha Manjula2
, Jaewon Kim3
, Jin Hyung Kim3
, Seonju Nam3
, Minjun Kim3
, Eunjin Cho1
, Mohammad Shamsul Alam Bhuiyan4
, Md. Abdur Rashid5
, Jun Heon Lee1,3,*
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1Department of Bio-AI Convergence, Chungnam National University, Daejeon 34134, Korea
2Department of Animal Science, Uva Wellassa University, Badulla 90000, Sri Lanka
3Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Korea
4Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
5Poultry Production Research Division, Bangladesh Livestock Research Institute, Dhaka-1341, Bangladesh |
Correspondence:
Jun Heon Lee, Tel: +82-42-821-5779, Email: junheon@cnu.ac.kr
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Received: 11 August 2023 • Revised: 18 October 2023 • Accepted: 15 December 2023 |
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| Abstract |
Objective
The major histocompatibility complex in chicken demonstrates a great range of variations within varities, breeds, populations and that can eventually influence their immuneresponses. The preset study was conducted to understand the major histocompatibility complex-B (MHC-B) variability in five major populations of Bangladesh native chicken: Aseel, Hilly, Junglefowl, Non-descript Deshi, and Naked Neck.
Methods
These five major populations of Bangladesh native chicken were analyzed with a subset of 89 single nucleotide polymorphisms (SNPs) in the high-density MHC-B SNP panel and Kompetitive Allele-Specific polymerase chain reaction genotyping was applied. To explore haplotype diversity within these populations, the results were analyzed both manually and computationally using PHASE 2.1 program. The phylogenetic investigations were also performed using MrBayes program.
Results
A total of 136 unique haplotypes were identified within these five Bangladesh chicken populations, and only one was shared (between Hilly and Naked Neck). Phylogenetic analysis showed no distinct haplotype clustering among the five populations, although they were shared in distinct clades; notably, the first clade lacked Naked Neck haplotypes.
Conclusion
The present study discovered a set of unique MHC-B haplotypes in Bangladesh chickens that could possibly cause varied immune reponses. However, further investigations are required to evaluate their relationships with global chicken populations. |
| Keywords:
Bangladesh Native Chicken; Haplotypes; Phylogeny; Kompetitive Allele Specific Polymerase Chain Reaction (PCR); Major Histocompatibility Complex-B |
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