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Association of histamine-N-methyl transferase gene polymorphisms with carnosine content in red-brown Korean native chickens |
Jean Pierre Munyaneza1 
, Minjun Kim1
, Eunjin Cho2
, Aera Jang3
, Hyo Jun Choo4,*
, Jun Heon Lee1,2,*
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1Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Korea
2Department of Bio-AI Convergence, Chungnam National University, Daejeon 34134, Korea
3Department of Applied Animal Science, College of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea
4Poultry Research Institute, National Institute of Animal Science, Pyeongchang 25342, Korea |
Correspondence:
Hyo Jun Choo, Tel: +82-10-2647-2888, Fax: +82-33-330-9529, Email: hyojy@korea.kr
Jun Heon Lee, Tel: +82-42-821-5779, Fax: +82-42-825-9754, Email: junheon@cnu.ac.kr
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Received: 29 December 2023 • Revised: 21 February 2024 • Accepted: 14 March 2024 |
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| Abstract |
Objective
Carnosine and anserine affect the meat flavor. The contents of carnosine and anserine in meat are affected by genetic and environmental factors. This study aimed to discover the single-nucleotide polymorphisms (SNPs) in the histamine-N-methyl transferase (HNMT) and histamine-N-methyl transferase-like (HNMT-like) genes and to associate them with the content of carnosine and anserine in Korean native chickenred brown line (KNC-R).
Methods
This study used a total of 384 birds (males, n = 192; females, n = 192) aged 10 weeks old, for genotyping HNMT and HNMT-like genes. One synonymous SNP (rs29009298C/T) of the HNMT gene was genotyped by polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) methods whereas four missense SNPs (rs734406537G/A; rs736514667A/G; rs15881680G/A and rs316765035T/C) of the HNMT gene, and one missense SNP rs737657949A/C of the HNMT-like gene were genotyped by PCR allele competitive extension (PACE) genotyping technology. Two-way analysis of variance of the R program was used to associate HNMT genotypes with the contents of carnosine and anserine in KNC-R chickens.
Results
There were significant associations (p<0.05) between the genotypes of the synonymous SNP:rs29009298C/T, missense SNP rs736514667A/G of the HNMT gene and the content of carnosine in KNC-Rs. This study also reported the sex effect on the carnosine content, where females had more content of carnosine compared to that of male KNC-R.
Conclusion
Two SNPs (synonymous: rs735769522C/T) and missense: rs736514667A/G) in the HNMT gene might be used as genetic markers in the selection and breeding of chickens with better taste and high-flavored meat. |
| Keywords:
Carnosine; HNMT gene; Korean native chicken; Meat Flavour; Single-nucleotide Polymorphism (SNP) |
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