|
|
|
Revealing the global mechanism related to carnosine synthesis in the pectoralis major of slow-growing Korat chickens using a proteomic approach |
Panpradub Sinpru1,a 
, Chanadda Suwanvichanee1,a
, Rujjira Bunnom1
, Satoshi Kubota1
, Jirawat Yongsawatdigul2
, Wittawat Molee1
, Kanjana Thumanu3
, Amonrat Molee1,*
|
1School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand
2School of Food Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand
3Synchrotron Light Research Institute (Public Organization), Nakhon Ratchasima, 30000, Thailand |
Correspondence:
Amonrat Molee, Tel: +66-44-22-4569, Email: amonrat@sut.ac.th
|
Received: 27 February 2024 • Revised: 30 March 2024 • Accepted: 15 May 2024
aThese authors contributed equally to this work. |
|
|
| Abstract |
Objective
This study aimed to find global mechanisms related to carnosine synthesis in slow-growing Korat chickens (KRC) using a proteomic approach.
Methods
M. pectoralis major samples were collected from 10-week-old female KRC including low-carnosine (LC, 2,756.6±82.88 μg/g; n = 5) and high-carnosine (HC, 4,212.5 ±82.88 μg/g; n = 5).
Results
We identified 152 common proteins, and 8 of these proteins showed differential expression between the LC and HC groups (p<0.05). Heat shock 70 kDa protein 8, Heat shock 70 kDa protein 2, protein disulfide isomerase family A, member 6, and endoplasmic reticulum resident protein 29 were significantly involved in protein processing in the endoplasmic reticulum pathway (false discovery rate<0.05), suggesting that the pathway is related to differential carnosine concentration in the M. pectoralis major of KRC. A high concentration of carnosine in the meat is mainly involved in low abundances of Titin isoform Ch12 and Connectin and high abundances of M-protein to maintain homeostasis during muscle contraction. These consequences improve meat characteristics, which were confirmed by the principal component analysis.
Conclusion
Carnosine synthesis may occur when muscle cells need to recover homeostasis after being interfered with carnosine synthesis precursors, leading to improved muscle function. To the best of our knowledge, this is the first study to describe in detail the global molecular mechanisms in divergent carnosine contents in meat based on the proteomic approach. |
| Keywords:
Carnosine Content; Molecular Function; Molecular Pathway; Proteome; Slow-growing Chicken |
|
>
PubReader
ePub Link
Full text via DOI
Full text via PMC
Download Citation
Supplement1
