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Nicotinamide benefited amino acid metabolism and rumen fermentation pattern to improve growth performance of growing lambs |
YuAng Wang1,a 
, Hao Wu1,a
, Yiwei Zhang1
, Mingfeng Fei2
, Zhefeng Li3
, Daxi Ren4
, Chong Wang1,*
, Xiaoshi Wei1,*
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1College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A & F University, Zhejiang, 311300, China
2Huzhou Zifeng Ecological Agriculture Co., Ltd, Huzhou, Zhejiang 313000, China
3Hangzhou King Techina Feed Co., Ltd, Hangzhou, Zhejiang 311107, China
4Institute of Dairy Science, College of Animal Science, Zhejiang University, Hangzhou, Zhejiang, 310058, China |
Correspondence:
Chong Wang, Tel: +86-571-63741751, Fax: +86-571-63741751, Email: wangcong992@163.com
Xiaoshi Wei, Tel: +86-571-63741751, Fax: +86-571-63741751, Email: deardoris163@163.com
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Received: 9 January 2024 • Revised: 20 March 2024 • Accepted: 15 May 2024
aThese authors contributed equally to this work. |
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| Abstract |
Objective
Nicotinamide (NAM) is easily degraded in the rumen, but the rumen-protected NAM (RPN) supplementation might enable the use of NAM in ruminants. This study aimed to elucidate the effects of RPN supplementation on growth performance, rumen fermentation, antioxidant status and amino acid (AA) metabolism in growing lambs.
Methods
A total of 128 healthy and similar lambs (21.3±0.28 kg, 70±6.3 days of age) were allotted to 1 of 4 groups. The treatments were 0, 0.5, 1, and 2 g/d RPN supplementation. The RPN products (50% bioavailability) were fed at 0700 h every day for 12 weeks. All lambs were fed the same pelleted total mixed rations to allow ad libitum consumption and had free access to water.
Results
The RPN tended to increase the average daily gain and feed efficiency. The tendencies of RPN×day interaction were found for dry matter intake during the entire study (p = 0.078 and 0.073, respectively). The proportions of acetic acid, isobutyric acid and isovaleric acid were decreased, whereas the proportions of propionic acid and valeric acid were increased (p<0.05). The ratio of acetic acid to propionic acid was decreased (p<0.05). Moreover, the antioxidative status was enhanced and the glucose concentration was increased by RPN (p<0.05). In addition, 17 AAs were detected in plasma, of which 11 AAs were increased by RPN (p<0.05). Plasma metabolomics analysis identified 1,395 compounds belonging to 15 classes, among which 7 peptides were significantly changed after RPN supplementation.
Conclusion
Overall, the results suggested that RPN supplementation favoured the rumen fermentation pattern to propionic acid-type with benefited glucose metabolism, enhanced antioxidant capacity, and changed the AA and small peptide metabolism. This study provides a new perspective for studying the relationship between vitamin and AA metabolism. |
| Keywords:
Amino Acid Metabolism; Antioxidant Capacity; Metabolomics Profile; Rumen Fermentation Pattern; Rumen-protected Nicotinamide |
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