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Identification of immune-associated genes with altered expression in the spleen of mice enriched with probiotic Lactobacillus species using RNA-seq profiling |
Anh Duc Truong1,a 
, Ha Thi Thanh Tran1,a
, Nhu Thi Chu1
, Lanh Phan1
, Hoai Thi Phan1
, Thu Huong Dang2
, Hoang Vu Dang2,*
, La Anh Nguyen2,*
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1Department of Biochemistry and Immunology, National Institute of Veterinary Research, Dong Da, Hanoi 100000, Vietnam
2Department of Microbial Biotechnology, Food Industries Research Institute, Thanh Xuan Distr., Hanoi 100000, Vietnam |
Correspondence:
Hoang Vu Dang, Tel: +84-919511688, Email: dangvuhoang@nivr.gov.vn
La Anh Nguyen, Tel: +84-904121294, Email: laanhnguyen@firi.vn
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Received: 29 April 2024 • Revised: 1 July 2024 • Accepted: 25 July 2024
aThese authors contributed equally to this work. |
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| Abstract |
Objective
Probiotics are living microorganisms that can provide health benefits when consumed. Here, we investigated the effects of probiotics on gene expression in the spleen of mice using RNA-sequencing analysis between negative control and probiotic groups (including 4 Lactobacillus strains: Lactobacillus fermentum, L. casei, L. plantarum, and L. brevis).
Methods
Mice exposed with probiotic in 4 weeks by intragastric administration. Then, spleen tissues of the control and probiotics groups were collected on days 14 and 28 for RNA sequencing.
Results
In total, 665, 186, and 81 differentially expressed genes (DEGs) were significantly expressed on day 14 vs control, day 28 vs control groups, and probiotics day 28 vs day 14 groups, respectively. On the other hand, 12 toll-like receptor genes underwent additional validation through quantitative real-time polymerase chain reaction (qRT-PCR), affirming the increased alignment between qRT-PCR and RNA-Seq findings. In addition, the Kyoto encyclopedia of genes and genomes and gene ontology analyses revealed that the DEGs were predominantly enriched in defense responses to pathogens, including inflammatory bowel diseases, malaria, leukaemia virus 1, and herpes virus, as well as immune processes related to immune response and signal transduction. This study represents the first investigation into mice's gene expression in the spleen exposed to probiotics using Lactobacillus spp. isolated from a field strain in Vietnam.
Conclusion
Our results provide valuable insights into the impacts and functions of probiotics on mammalian development, offering crucial information for the potential therapeutic use of probiotics in defending against pathogens in Vietnam. The findings from this study highlight the potential of probiotics in modulating gene expression in the spleen, which may have implications for immune function and overall health in mice. |
| Keywords:
Differentially Expressed Gene; Immune Response; Lactobacillus spp.; Probiotic; Signaling Pathway |
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