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Transcriptome and targeted metabolome analysis of lipid profiles, nutrients compositions and volatile compounds in longissimus dorsi of different pig breeds |
Zhen Luo1,2 
, Ting Lai1
, Yijia Fan1
, Chengbin Yu1
, Wei Li2
, Meng Li3
, Shenghui Lei4
, Jing Zhang1
, Weina Xu1
, Zhe Wang1,*
, Jianxiong Xu1,*
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1Shanghai Key Laboratory of Veterinary Biotechnology/Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China
2Key Laboratory of Molecular Animal Nutrition, Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou, China
3Jinan Laiwu Pig Industry Technology Research Institute Co., Ltd., Laiwu, China
4Shanghai Songjiang Center for Animal Disease Control and Prevention, Songjiang, China |
Correspondence:
Zhe Wang, Tel: +86-13361936206, Email: wangz@sjtu.edu.cn
Jianxiong Xu, Tel: +86-21-34205767, Email: jxxu1962@sjtu.edu.cn
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Received: 9 August 2024 • Revised: 26 August 2024 • Accepted: 10 October 2024 |
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| Abstract |
Objective
Improving meat quality is important for commercial production and breeding. The molecular mechanism of intramuscular fat (IMF) deposition and meat characteristics require further study.
Methods
This study aimed to study the mechanism of IMF deposition and meat characteristics including redox potential, nutrients compositions and volatile compounds in longissimus dorsi (LD) by comparing with different pig breeds including Shanghai white (SW), Duroc×(Landrace Yorkshire) (DLY) and Laiwu (LW) pigs.
Results
Results showed that the contents of IMF, triglyceride (TG), total cholesterol (TC), and redox potential parameters were lower, while the content of malondialdehyde (MDA) and activity of lactate dehydrogenase (LDH) were higher in LD of SW pigs compared with LW pigs (p<0.05). No differences were observed about these parameters between SW and DLY pigs. Also, the contents of medium-long chain fatty acids and γ-aminobutyric acid (GABA) were higher, while Asp was lower in LD of SW pigs compared with LW pigs (p<0.05). Volatile compounds results showed that 6 ketones, 4 alkenes, 11 alkanes, 2 aldehydes, 1 alcohol were increased, and cholesterol was decreased in SW pigs compared with LW pigs. Transcriptome results showed that differential expressed genes involved in lipid synthesis, metabolism and transport in LD between SW and LW pigs, which were further verified by quantitative polymerase chain reaction. Spearman correlation showed that HSL and Nedd4 were positively related to contents of TG and IMF, while negatively related to volatile compounds and fatty acids (p<0.05). Plin3 and Mgll were negatively related to contents of TG, IMF and cholesterol, while positively related to MDA, LDH, and volatile compounds (p<0.05). PPARA was negatively related to contents of TC and IMF, and activity of superoxide dismutase, while positively related to volatile compounds (p<0.05).
Conclusion
Our study provided new insights into potential mechanisms of IMF deposition, nutrients composition and volatile compounds of muscular tissues of different pig breeds. |
| Keywords:
Intramuscular Fat; Lipid Profiles; Longissimus Dorsi; Pig Breeds; Volatile Compounds |
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