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Combined transcriptome and metabolome analysis of chicken follicles in Tengchong Snow Chicken follicle selection |
Yanli Du1 
, Xiannian Zi4
, Kun Wang4
, Jinshan Ran1
, Hanyi Xiang2
, Li Lei2
, Yong Liu2,3,4,*
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1College of Agronomy and Life Sciences, Kunming University, Kunming 650200, China
2Yunnan Rural Revitalizing Education Institute, Yunnan Open University, Kunming 650101, China
3Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Guangxi Bufialo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China
4College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China |
Correspondence:
Yong Liu,Email: liuyong@ynou.edu.cn
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Received: 9 December 2024 • Revised: 10 January 2025 • Accepted: 3 March 2025 |
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| Abstract |
Objective
The development of pre-hierarchical follicles (PHFs), especially small yellow follicles (SYFs), directly affects the recruitment of dominant follicles and thus affects the egg-laying performance of chickens. However, the development of PHFs, especially SYFs, and their regulatory mechanism remain unclear.
Methods
We performed transcriptomic and metabolomic analyses of large white follicles (LWFs) and SYFs in chickens.
Results
Transcriptome sequencing revealed 258 differentially expressed genes (DEGs) between SYFs and LWFs, of which 172 were upregulated and 86 were downregulated. The DEGs were mapped to 17 KEGG pathways, including Glutathione metabolism, Ferroptosis, Calcium signaling pathway, and Neuroactive ligand–receptor interactions, etc. The metabolome analysis revealed 129 significant differential metabolites (DMs), including 36 upregulated DMs and 93 downregulated DMs. The DMs were mapped to nine KEGG pathways, including Glutathione metabolism, alpha-Linolenic acid metabolism, and Linoleic acid metabolism, etc. The combined transcriptional and metabolic analysis revealed significantly enriched pathways. Five KEGG pathways associated with follicular development were identified, including Glutathione metabolism, Ferroptosis, alpha-Linolenic acid metabolism, Linoleic acid metabolism, and Pyrimidine metabolism.
Conclusion
Glutathione metabolism may directly inhibit Ferroptosis, which can induce apoptosis of granulosa cells (GCs), thereby regulating SYFs development in chickens. These findings provided a reference for improving the egg-laying performance of Tengchong snow chickens. |
| Keywords:
Egg-laying chickens; Follicle; Transcriptome analysis; Reverse transcription qPCR; Metabolome analysis |
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