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Effects of Mongolian ‘Bergenia crassifolia L. (root)’ extract on rumen methane emission reduction and microbial community |
Xinrui Zhao1 
, Otgonpurev Sukhbaatar1,2
, Linlin Kou1
, Xinming Cheng1
, Metha Wanapat3
, Mahmoud Kamal1,4
, Togtokhbayar Norovsambuu5
, Zhanying Sun1,*
, Yanfen Cheng1
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1Laboratory of Gastrointestinal Microbiology, National Center for International Research on Animal Gut Nutrition, Nanjing Agricultural University, Nanjing, China
2Department of Chemistry, School of Applied Sciences, Mongolian University of Life Sciences, Ulaanbaatar, Mongolia
3Tropical Feed Resources Research and Development Center, Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand
4Animal Production Research Institute, Agricultural Research Center, Dokki, Giza, Egypt
5Department of Animal nutrition and Grassland, School of Animal Sciences and Biotechnology, Mongolian University of Life Sciences, Ulaanbaatar, Mongolia |
Correspondence:
Zhanying Sun, Tel: +86-025-84395523, Fax: +86-025-84395523, Email: sunzhanying@njau.edu.cn
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Received: 28 November 2024 • Revised: 26 February 2025 • Accepted: 24 April 2025 |
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| Abstract |
Objective
The study investigates the effects of Mongolian plants on reducing methane emissions, fermentation parameters, and microbial communities in grazing systems.
Methods
Initially, various Mongolian plants were screened to identify those that effectively reduced methane production in vitro. The most effective plant was selected for the extraction of its active components, resulting in a solid extract with a quantified concentration of its bioactive compounds. Extracts of 0, 1.0, 2.0, 3.0, 4.0, and 5.0% of DM were added, respectively, and then subjected to in vitro rumen fermentation trials using rice straw and alfalfa as substrates to evaluate their methane reduction effects. Key metrics assessed included gas production, methane production, and rumen fermentation parameters. After the fermentation experiments, we extracted DNA from the rumen fluid for quantitative PCR and 16S high-throughput sequencing analysis.
Results
Bergenia crassifolia L. (root) was identified as the plant exhibiting the most significant methane reduction capability with approximately 70% condensed tannins in its extract. This extract significantly decreased methane production in the rice straw substrate (p<0.01), while only the highest dose had a notable effect in the alfalfa substrate (p<0.01). Furthermore, low doses of the extract did not significantly impact rumen fermentation parameters. The genera Ruminococcus and Christensenellaceae R-7 were identified as the principal contributors to the reduction of methane emissions (p<0.01).
Conclusion
Bergenia crassifolia L. (root) was more effective in reducing methane emissions from low-quality roughage compared to high-quality roughage, with an optimal addition concentration of 1.0% for low-quality feed. This plant presents a promising new strategy for mitigating rumen methane emissions in grazing systems. |
| Keywords:
Bergenia crassifolia L. (root); Tannin; Methane; in vitro fermentation |
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